ABSTRACT
Aim: The aim of the present study was to evaluate the different doses of Butyric acid [BA] and Arsenic [As] in liver mitochondria oxidative stress and pancreatic islet insulin secretion of male mouse
Background: BA is found in many foods and As as a toxic metal is present in drinking water. They can induce oxidative stress in tissues
Methods: In this experimental study, Liver mitochondria were isolated by administration of the different centrifugation method and pancreatic islets were isolated by collagenase method. Mitochondria were incubated by BA [35, 75, 150, 300 microM] and As [20, 50, 100, 200 microM] as the islets were incubated by BA [250, 500, 1000, 1500 microM] and As [50, 100, 200 microM] for 1 hour. At the end of the experiment, mitochondrial viability and membrane potential, ROS, MDA, GSH and islets insulin secretion were measured by their specific methods
Results: BA and As administration increased mitochondrial levels of ROS, MDA and decreased GSH and pancreatic islet insulin secretion in a dose dependent manner [p<0.05]. The doses of BA 75microM and As 100microM have been revealed the most mitochondria toxic concentrations. Also, the doses of 1000microM for BA and 100microM for As were considered as reducing concentrations for islets insulin secretion. Additionally, co administration of them intensified more these effects
Conclusion: Alone or in combination administration of BA and As induced oxidative stress in liver mitochondria and decreased insulin secretion of pancreatic islets